Şamlıoğlu was born on March 20, 1956 in Istanbul. His mother's name is Nezahat and his father's name is Servet Şamlıoğlu. He has one sister and one brother. His father served as the 1st President of the Court of Accounts between November 20, 1984 and April 20, 1991.

In 1974, she started studying at Istanbul University, Faculty of Journalism and Public Relations. She left her studies midway and went to Germany and started to study languages in Frankfurt. He also participated in amateur theater groups. When he returned to Turkey, he started to study at METU Faculty of Architecture. But he dropped out again.

He worked at Anadolu Agency for a year. She also did drawing work at an advertising agency. Then he graduated from Ankara Hacettepe University Ankara State Conservatory, Department of Theater. In 1982, he started working as an actor and assistant director at Adana State Theater.

Between 1988 and 1989, he taught theater courses at Hacettepe University State Conservatory, Department of Theater, and served as the Secretary General of the State Theaters for a period.

Written by Ayşenil Şamlıoğlu in 1993, the book "Diplomacy of Lunacy" was scripted, directed and filmed by Tunç Başaran in 1998.

In 1993, she starred in the TV series "Ferhunde Hanımlar" with Beyhan Saran, Baykal Saran, Güven Hokna, Melek Baykal, Şahap Sayılgan, İpek Çeken, Hatice Aslan, Tamer Karadağlı until 1999.

Between 2008 and 2009, she played the character of Hafize in the TV series "Yol Arkadaşım", written by Çağan Irmak and starring Özge Özberk.

On May 29, 2009, he was appointed as the General Artistic Director of Istanbul City Theaters. In 2013, he served as a jury member at the 17th Afife Theater Awards.

In 2014, she starred in the TV series "My Husband's Family". In the same year, she starred in the movie "Eyyvah Eyvah 3" starring Ata Demirer and Demet Akbağ, along with Özge Borak, Serra Yılmaz, Salih Kalyon, Tarık Ünlüoğlu.

In 2017, he played the character Meyro in the movie "Ay Lav Yu Tuu" directed and written by Sermiyan Midyat. The cast of the movie, which is the second installment of the 2010 comedy "Ay Lav Yu Tuu" series, includes, in addition to Sermiyan Midyat, Nikki Leigh, Ayça Nehirgacı, Steve Guttenberg, Josh Folan, Barry Corbin, Ayşenil Şamlıoğlu, Natalie Burn, Kevork Malikyan, Gizem Karaca and Bora Akkaş.