Güzin Özyağcılar was born on December 7, 1945 in Bursa. Since her father was a military officer, she spent her childhood in various provinces of Anatolia. In 1960, when her father retired, they moved to Istanbul. She attended high school at Istanbul High School for Girls. While in high school, she acted in the theater play "Cimri". After high school, she entered the Istanbul Municipality Conservatory Theater Department.

He graduated from the Istanbul Municipality Conservatory Theater Department in 1968. He went to London as soon as he graduated in 1968, returned in 1970 and became a professional with the play 'Yaygara 70' at Dormen Theater.

Later, he worked in many private theaters such as Ulvi Uraz and then in the Gazete Theater, which he founded with Erdal Özyağcılar Haşmet Zeybek and Ertuğ Koruyan. In 1974, he joined the Istanbul Metropolitan Municipality Theaters, where he still works today.

In 1989, she played the role of "Serpil", the wife of Salih Kalyon, nicknamed "tak tak Sedat", in the series "Bizimkiler".

In 2010, she appeared in the series "Karadağlar", based on the novel "The Brothers Karamazov" by Russian writer Dostoevsky, together with her husband Erdal Özyağcılar and daughter Zeynep. İbrahim Çelikkol, Korel Cezayirli, Burak Sağyaşar, Ahmet Rıfat Şungar, Hatice Şendil and Aliona Bozbey also starred in the series.

Güzin Özyağcılar married theater and cinema actor Erdal Özyağcılar in 1972, whom she met when she was a conservatory student. She has a son named Emrah and a daughter named Zeynep Özyağcılar.